Coding

Part:BBa_K4447001:Design

Designed by: Rafael Garcia Gomez   Group: iGEM22_TecMonterrey_GDL   (2022-09-24)


EryK coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1197
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.

Source

The original sequence was reported by Stassi et al. (1993). Sequence can be obtained through GenBank (L05776).

References

[1]. Stassi, D., Donadio, S., Staver, M. J., & Katz, L. (1993). Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. Journal of bacteriology, 175(1), 182–189. https://doi.org/10.1128/jb.175.1.182-189.1993